My experience is that whenever I autoclave a starter I get boil over. The starters are exactly half the volume of their flask (e.g. 1L starter in a 2L flask). I have tried various things to overcome this:
1. Leave the prepared starter on the stir plate until gas quits evolving (takes approx 30 min).
2. Gently heat the starter before autoclaving.
3. Lower the temperature slowly upon completion of the sterilization cycle. tono de llamada gratis: https://descargartonos.info/
Nothing seems to work. I still get boilover in the autoclave. The typical result is that a 1L starter (2L flask) will be reduced by 75-150 ml, and a 2L starter (4L flask) will be reduced by about the same amount.
Is there a fix for this, or am I forever condemned to cleaning the equipment after sterilizing a starter? (please don't mention catch pans. that isn't a fix!)
https://www.youtube.com/@Tonoparacecular
1. Leave the prepared starter on the stir plate until gas quits evolving (takes approx 30 min).
2. Gently heat the starter before autoclaving.
3. Lower the temperature slowly upon completion of the sterilization cycle. tono de llamada gratis: https://descargartonos.info/
Nothing seems to work. I still get boilover in the autoclave. The typical result is that a 1L starter (2L flask) will be reduced by 75-150 ml, and a 2L starter (4L flask) will be reduced by about the same amount.
Is there a fix for this, or am I forever condemned to cleaning the equipment after sterilizing a starter? (please don't mention catch pans. that isn't a fix!)
https://www.youtube.com/@Tonoparacecular
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